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Image Search Results
Journal: Methods in enzymology
Article Title: Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S
doi: 10.1016/bs.mie.2021.10.023
Figure Lengend Snippet: Three approaches, using different building blocks for introducing Sec, for synthesizing selenopeptides. Left: Fmoc-(S)-4-methoxybenzyl selenocysteine, Sec is introduced with side chain protection. Middle: Bis-Fmoc-selenocystine, Sec is introduced without side chain protection. Right: Fmoc-chloroalanine, Sec is introduced by replacing a chloroalanine in the synthesized peptide. In this chapter, we describe SPPS with Fmoc-Mob-selenocysteine (left).
Article Snippet: Materials for selenopeptide synthesis, purification, and quantification Dichloromethane (DCM) Dimethylformamide (DMF) Acetonitrile (ACN) N,N -Diisopropylethylamine (DIEA, or DIEPA) Piperidine 1-Hydroxy-7-azabenzotriazole (HOAT) 2,4,6-Trimethylpyridine (collidine, or TMP) 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b] pyridinium-3-oxide hexafluorophosphate (HATU) Trifluoroacetic acid (TFA) 2,2’-Dithiobis (5-nitropyridine) (DTNP) Triisopropyl silane (TIPS) 2-Chlorotrityl chloride resin from Chem-Impex Fmoc-glycine from
Techniques: Synthesized